The Development and Validation of Fast and Robust Stability Indicating RP-HPLC Method for Simultaneous Estimation of Azilsartan Medoxomil and Cilnidipine in Pharmaceutical Dosage Form

Objectives: A selective, precise and accurate RP-HPLC stability indicating assay method has been developed for the simultaneous estimation of Azilsartan medoxomil Cilnidipine in tablet dosage form. Materials Methods: The efficient chromatographic separation drug was achieved by using C18 (150mm×4.6mm, Agilent 5μm) Column at ambient temperature. Mobile phase contains triethylamine buffer (pH 3.5 adjusted with ortho-phosphoric acid) acetonitrile (40:60 V/V). Flow rate mobile 1.0 ml/min isocratic mode .Wavelength selected 249 nm photo diode array detector. Results: retention time peak 1, 2 were noticed to be 2.16 min, 3.90 min 9.52 respectively. linearity range found 50 -150 μg/ml 12.5-37.5 percent recoveries 99.27±0.58 98.65±0.49 Various stress testing conditions such applied ingredients formulation. degradants drugs efficiently separated enhanced conditions. validated as per recommendation parameters International council on harmonization guideline Q2(R1). Conclusion: validation stated that substances from its can routinely formulation a laboratory.